Lab Unlimited UK
The Carl Stuart Group was founded in 1984 by Stuart and Gillian Smith. From a small beginning our business has grown to encompass many services and products, covering different market sectors and geographical regions. The focus of our business, however, remains the same. We are a customer orientated company and strive to meet and exceed your expectations in everything we do.
As our portfolio grew we decided to present and promote our product and technical service offerings under distinct brands. The Laboratory instrumentation, consumables and technical & integration services are promoted under Lab Unlimited.
The Lab Unlimited product portfolio offers laboratory/analytical products from more than four hundred leading manufacturers. A substantial part of this offering is published on this very site. We also produce a printed catalogue that can be requested by emailing us.
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Canvax Clean-Easy PCR Purification Kit AN0064 - DNA Spin Column based Purification
Clean-Easy PCR Purification Kit, for highly efficient, consistent & rapid purification of dna and remove contaminants from reaction mixtures CleanEasy PCR Purification Kit provides an highly efficient, consistent and rapid method to purify DNA and remove contaminants from reaction mixtures (e.g. PCR, digestion or labeling reactions). Comfortable CleanEasy MiniSpin Columns contains an exclusive membrane that allows DNA adsorption in presence of chaotropic salts and the removal of contaminants. Main Feautres: Really fast and easy procedure: it takes 14 minutes to get results with minimal handling steps. Reproducible yields: high DNA Recovery (70-90%) and consistent yields of pure DNA. Sensitive: proven performance for DNA fragments as short as 75 bp. Pure genomic DNA: ready-to-use in all Molecular Biology applications. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes for 50 rxn: - 50 CleanEasy MiniSpin Columns - 50 Collection tubes - 25 mL PB Buffer - 11.25 mL PE Buffer - 9 mL EB Buffer Applications: Removal of proteins and salts from PCR, restriction digestion, dephosphorylation, ligation or labelling reactions. Changing of a restriction enzyme buffer. Re-purification of genomic DNA.Request a Quote Supplied by Lab Unlimited UK -
Canvax CVX5a Chemically Competent Cells C0031 - Related Compounds
CVX5a Chemically Competent Cells, versatile, convenient & cost-effective solution for routine subcloning procedures CVX5a Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. CVX5a Genotype: F - , gyrA96, recA1, endA1, thi1, hsdR17 (rK - mK +), deoR, supE44, = (lacZYA-argF) U169 f80lacZ=M15 (similar to DH5 ). Main Feautres: Versatile: proven performance for high-efficiency transformation in a wide variety of applications. Convenient: ideal for routine. Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. Cost avoidance: dry ice free of charge. Includes: Includes for 40 rxn: - 2,000 µL CVX5a (1 x 107 CFU/µg) - 10 µL pUC18 Transformation Control Plasmid (10 ng/µL) - 50 mL SOC Medium - Dry ice Applications: Routine cloning and subcloning of genes into plasmid vectors.Request a Quote Supplied by Lab Unlimited UK -
Canvax CVX5a Chemically Competent Cells C0032 - Related Compounds
CVX5a Chemically Competent Cells, versatile, convenient & cost-effective solution for routine subcloning procedures CVX5a Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. CVX5a Genotype: F - , gyrA96, recA1, endA1, thi1, hsdR17 (rK - mK +), deoR, supE44, = (lacZYA-argF) U169 f80lacZ=M15 (similar to DH5 ). Main Feautres: Versatile: proven performance for high-efficiency transformation in a wide variety of applications. Convenient: ideal for routine. Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. Cost avoidance: dry ice free of charge. Includes: Includes for 40 rxn: - 2,000 µL CVX5a (1 x 107 CFU/µg) - 10 µL pUC18 Transformation Control Plasmid (10 ng/µL) - 50 mL SOC Medium - Dry ice Applications: Routine cloning and subcloning of genes into plasmid vectors.Request a Quote Supplied by Lab Unlimited UK -
Canvax CVX5a Chemically Competent Cells C0033 - Related Compounds
CVX5a Chemically Competent Cells, versatile, convenient & cost-effective solution for routine subcloning procedures CVX5a Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane. CVX5a Genotype: F - , gyrA96, recA1, endA1, thi1, hsdR17 (rK - mK +), deoR, supE44, = (lacZYA-argF) U169 f80lacZ=M15 (similar to DH5 ). Main Feautres: Versatile: proven performance for high-efficiency transformation in a wide variety of applications. Convenient: ideal for routine. Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. Cost avoidance: dry ice free of charge. Includes: Includes for 40 rxn: - 2,000 µL CVX5a (1 x 107 CFU/µg) - 10 µL pUC18 Transformation Control Plasmid (10 ng/µL) - 50 mL SOC Medium - Dry ice Applications: Routine cloning and subcloning of genes into plasmid vectors.Request a Quote Supplied by Lab Unlimited UK -
Canvax DNA Polymerase I, Large (Klenow) Fragment P0041 - Related Polymerases
DNA Polymerase I, Large (Klenow) Fragment, large fragment of dna polymerase i (10 u/µl) Klenow Fragment is the Large Fragment of DNA Polymerase I. It shows 5'=3' polymerase activity and 3'=5' exonuclease (proofreading) activity, but lacks 5'=3' exonuclease activity of DNA Polymerase I. Main Feautres: Recombinant: isolated from E. coli cells with a cloned fragment of the polA gene. DNase I-dependent nick translation: second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs. Generates probes using random primers. Creates blunt ends. Complete solution: supplied with 10x Reaction Buffer. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes for 500 U: - 50 µL Klenow Fragment (10 U/µL) - 1 mL Reaction Buffer (10x) Applications: DNA sequencing, fill-in of 5’ overhangs and removal of 3’ overhangs to form blunt ends and second strand synthesis in mutagenesis. DNA blunting by fill-in of 5’-overhangs or removal of 3‘-overhangs. Random-primed DNA labeling. Labeling by fill-in 5’-overhangs of dsDNA. DNA sequencing by the Sanger method. Site-specific mutagenesis of DNA with synthetic oligonucleotides. Second strand synthesis of cDNA.Request a Quote Supplied by Lab Unlimited UK -
Canvax DNase I EZ0018 - Active Enzymes
DNase I, free of rnases. DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends. It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2= and Ca2=) for maximal activity. Main Feautres: Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer). High specific activity: more than 2,000 Kunitz units per mg protein. High Quality: free of RNase and Proteinase contamination. Complete solution: supplied with 10x Reaction Buffer. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes : - 1 mL DNase I (10 mg/mL) - 1 mL Reaction Buffer (10x) * 10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2 Applications: Removal of residual genomic DNA from RNA samples. Degradation of DNA template in transcription reactions. DNAse I footprinting. Perform Nick Translation.Request a Quote Supplied by Lab Unlimited UK -
Canvax DNase I EZ0019 - Active Enzymes
DNase I, free of rnases. DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends. It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg2= and Ca2=) for maximal activity. Main Feautres: Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer). High specific activity: more than 2,000 Kunitz units per mg protein. High Quality: free of RNase and Proteinase contamination. Complete solution: supplied with 10x Reaction Buffer. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes : - 1 mL DNase I (10 mg/mL) - 1 mL Reaction Buffer (10x) * 10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2 Applications: Removal of residual genomic DNA from RNA samples. Degradation of DNA template in transcription reactions. DNAse I footprinting. Perform Nick Translation.Request a Quote Supplied by Lab Unlimited UK -
Canvax Exonuclease I (E. coli) EZ0016 - Active Enzymes
Exonuclease I (E. coli), from e. coli (20 u/µl) Exonuclease I, the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3'=5' direction releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Main Feautres: High Specific Activity: 185,000 U/mg. 3' -> 5' single strand exonuclease. Versatile: suitable with a wide-range of Buffer conditions. Compatible with Magnesium. Complete solution: supplied with 10x Exonuclease I Reaction Buffer. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes for 5,000 U: - 5,000 U Exonuclease I (20 U/µL) - 375 µL Exonuclease I Reaction Buffer (10x) * 10x Reaction Buffer: 670 mM Glycine-KOH, 67 mM MgCl2, 100 mM 2-mercaptoethanol (pH 9.5, 25 °C). Applications: Removal of residual ssDNA, including oligonucleotides, from reaction mixes.Request a Quote Supplied by Lab Unlimited UK -
Canvax Exonuclease I (E. coli) EZ0017 - Active Enzymes
Exonuclease I (E. coli), from e. coli (20 u/µl) Exonuclease I, the product of the sbcB gene of E. coli, is an exodeoxyribonuclease that hydrolyzes single-stranded DNA (ssDNA) stepwise in a 3'=5' direction releasing 5'-mononucleotides and leaving the terminal 5'-dinucleotide intact. Main Feautres: High Specific Activity: 185,000 U/mg. 3' -> 5' single strand exonuclease. Versatile: suitable with a wide-range of Buffer conditions. Compatible with Magnesium. Complete solution: supplied with 10x Exonuclease I Reaction Buffer. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes for 5,000 U: - 5,000 U Exonuclease I (20 U/µL) - 375 µL Exonuclease I Reaction Buffer (10x) * 10x Reaction Buffer: 670 mM Glycine-KOH, 67 mM MgCl2, 100 mM 2-mercaptoethanol (pH 9.5, 25 °C). Applications: Removal of residual ssDNA, including oligonucleotides, from reaction mixes.Request a Quote Supplied by Lab Unlimited UK -
Canvax FastCONTROL p2LVa-SEAP/=NGFR - Lentiviral Dual Reporter PC0142 - Lentiviral Plasmids
FastCONTROL p2LVa-SEAP/=NGFR - Lentiviral Dual Reporter, for a faster, convenient & flexible target cell transfection control FastCONTROL Dual Reporters are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL p2LVa-SEAP/ DNGFR Dual Reporter is a Lentiviral vector that contains the human elongation factor 1 alpha promoter (PEF1a) to drive the expression of both secreted embryonic alkaline phosphatase (SEAP) and truncated nerve growth factor receptor (DNGFR). It is a HIV based lentivector, self-inactivating (SIN), lacking viral promoter and enhancer activity in its 3' long terminal repeat (LTR). Main Feautres: Fast: available combination of 2 cell location markers in one vector. Convenient: available in Lentiviral, Retroviral or non-viral vectors, for immediate in vivo and in vitro expression. Compatible with transient or stable transfections. Flexible: available with different mammalian resistance markers and Dual Reporter genes combination for different cell location. Risk-free: product covered by our Quality 100% Guarantee. Includes: Includes for 100 assays: - 15 µL FastCONTROL Dual Reporter (1 µg/µL) Plus Version - Includes for 100 assays: - 15 µL FastCONTROL Dual Reporter (1 µg/µL) - 0.2 mL CANFAST Transfection Reagent (1 mg/mL) - 15 µL pASSEMBLE Viral Packaging Vector (1 µg/ µL)æ æ ecotropic (tropism for rat or mouse cells) or amphotropic (tropism for most mammalian cells; hamster are not included). Applications: Control for assessing the efficiency of transfection in mammalian cells. Targering of different cell locations. Obtention of cell lines with reporter proteins.Request a Quote Supplied by Lab Unlimited UK
