Petri Dishes
Petri dishes are shallow, circular, transparent dishes with flat lids for the culture of microorganisms. They have rings or slots on the lids and bases so they are easy to stack. Multiple Petri dishes can be placed in one plastic container to create a 'multi-well plate'. They can be different sizes but the most common are 100mm in diameter by 15mm in height or 60mm in diameter. Petri dishes can be made of two types of glass. The first is borosilicate which is more expensive but less easily broken. The second glass type is made of flint which can be easily broken when heating, cooling or when undergoing rapid temperature changes. Some laboratories prefer to buy pre-sterilised dishes which are less expensive but are for one use only. There is a risk of cross contamination if they are reused. Glass types can be sterilised at a temperature of 120 degrees centigrade for at least fifteen minutes. In microbiological laboratories, Petri dishes are used as agar plates; they are partially filled with a warm liquid containing agar and other materials including nutrients, blood, salts, carbohydrates, dyes, indicators, amino acids or antibiotics. They are also used for eukaryotic cell culture and plant germination.
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